Journal: iScience

Article Title: Soap application alters mosquito-host interactions

doi: 10.1016/j.isci.2023.106667

Figure Lengend Snippet: Soap application can modulate mosquito attraction (A) Behavioral preferences of adult Aedes aegypti females between two clean sleeves (light gray) or between a human-worn nylon sleeve and clean sleeves. A dual-choice assay was used for the behavioral experiments in which mosquitoes are released into a test cage and can choose to land on either of the test or the control sleeve (See Figure 1 A for details). Top: A preference index was calculated based on the number of landings on each sleeve (see methods for details). Asterisks indicate distributions of landings that were significantly different from chance ( i.e., 50% on each sleeve; Binomial Exact test, p < 0.05). Bottom: Proportion of mosquitoes landing on either sleeve. Asterisks denote a significant difference from the proportion of landings when two clean sleeves are presented (i.e., 0.225; Binomial Exact test, p < 0.05). (B) Top: Behavioral preferences of mosquitoes when provided with a choice between a sleeve worn on the unwashed arm and a sleeve worn on the washed arm of the same volunteer (colored as a function of the soap brand). Asterisks indicate distributions of landings that were significantly different from chance ( i.e., 50% on each sleeve; Binomial Exact test, p < 0.05). Bottom: Proportion of mosquitoes landing on either sleeve. Asterisks denote a significant difference from the proportion of landings observed when only the unwashed sleeve is presented for the corresponding volunteer ( i.e. , 0.57 for volunteer 1, 0.53 for volunteer 2, 0.40 for volunteer 3, and 0.48 for volunteer 4; Binomial Exact test, p < 0.05). The volunteer identity is represented with the same point shape as in Figure 2 . Across treatments: 35 < n < 78. C) Top: Behavioral preferences of mosquitoes when provided a choice between two sleeves worn by the same individual host either unaltered (gray bar) or spiked with 1 mL of an attractive mixture (yellow bar) or an aversive mixture (red bar). Asterisks indicate distributions of landings that were significantly different from chance ( i.e., 50% on each sleeve; Binomial Exact test, p < 0.05). Bottom: Proportion of mosquitoes landing on either sleeve. Asterisks denote a significant difference from the proportion of landings when two unwashed sleeves are presented ( i.e. , 0.53; Binomial Exact test, p < 0.05). Across treatments: 100 < n < 132. Error bars for both the preference index and the proportion of landings represent the standard error to the mean.

Article Snippet: Mosquitoes: Aedes aegypti Rockefeller strain , Manassas, VA, USA , Rockefeller strain, MR-734, MR4, ATCC®.

Techniques: Control

Journal: iScience

Article Title: Soap application alters mosquito-host interactions

doi: 10.1016/j.isci.2023.106667

Figure Lengend Snippet:

Article Snippet: Mosquitoes: Aedes aegypti Rockefeller strain , Manassas, VA, USA , Rockefeller strain, MR-734, MR4, ATCC®.

Techniques: Recombinant, Software, Combined Bisulfite Restriction Analysis Assay, Mass Spectrometry